Summary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells.The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS.Here, we describe Contribuições da estratégia de ensino estudo de caso para o desenvolvimento de habilidades didáticas em licenciandos de Química a methodology to quantitatively measure ROS in ex vivo mouse brain slices at baseline and after neural stimulation.
Evaluating ROS in Sovereign CDS Premiums’ Reaction to Macroeconomic News: An Empirical Investigation slices provides a more complete picture of neural redox signaling than when measured in isolated neurons or astrocytes.For complete details on the use and execution of this protocol, please refer to Vasavda et al.(2019).